PX’Monoclonals will offer services ranging from the antigen generation
through to antibody purification for research (immunohistochemistry,
FACS, ELISA assay, Western/Dot-blotting) or therapeutic purposes
(development of neutralizing antibodies). According to the project
specifications and objectives, the platform will propose some or
all of the following steps:
• Production
of the immunogen
• Animal
immunization
- from
DNA, transfected cells or purified protein
- use
of standard or custom immunization protocols
• Fusion
et generation of hybridomas
• Screening
of hybridomas
- Primary
and secondary screening
• Cloning
of the hybridomas of interest by limited dilution
• Characterization
of antibody
• In
vitro production
• Purification
of antibody
- Immunoaffinity,
size exclusion, ion exchange chromatography
• Antibody
labelling
- Current
labelling (biotine, peroxydase, fluorophore, etc.)
• Clone
storage in liquid nitrogen
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